EGFP expression in the midbrain (neurons, MHB), hindbrain (neurons), spinal cord (neurons), jaws, mouth, ionocytes and corpuscles of Stannius.

Genomic location: Chromosome 11

The insertion is in exon of gdf11 (growth differentiation factor 11)

DNA sequence flanking Tol2 insertion site

5:CTTATTTCTCTGGGACTGTCCGGAAGCGATGAGCCGAACCTCTTTCTAGC

CCCACTGAGCGAGATGTCCTCTGATATTGGAGTTTCTCTCTTTGACGTGGAT

GATGTGGAGTCTTCTGAGTGTTCAGCGTGCGTGTGGAGAGAACAGAGTAAAG

TCTTGCGACTGGAAACAATTAAATCACAAATACTTAGCAAACTGCGCTTAAA

ACAAGCACCCAACATCAGCAAAGAGGTGGTTAATCAGCTTCTTCCCAAAGCA

CCTCCGCTGCAGCAGCTGTTGGACCATCATGACTTCCAAGGGGACGCATCTT

CCTTAAAGGATTTCATAGACGCCGATGAGTATCACGCCACCACCGAGTCCGT

CATCACAATGGCTTCAAAGCGTGAGTTACAAAACTTCAAGCTTCAAGCTATC

TTTGCTTAAAATGCATAACTGATGTCTGTTTCATTCACAACACTCTCGCTCC

AATTTTCTTTTTTCCTTCAATCGCATTTACACAACTGTTTTGCACAGTTAAA

GGACAGCCACAAATATGCCACAGCATCTTTACAAACCCTTAAAGGATGACAT

GAAAGAGAAAAATGTGTTTTACGTAATTTACAAATATTTTTCAAATACAATA

ATACACATAGCCTATGATGCGAGATCTCTAACCATTTATGAAAGCCGTCTTT

GTTGCGCCTGTTACTGTTTACTTGAAAATAATCAATATCTTTAGGCAGTTGC

ATTGAATGTGGTTTACATTTACGTTATGCTAGTTACAAAAACAACAACAACA

ACAACAACAACAACAACGGAGAGCTATACAATATTTTTATATAATAAATGGT

GCACAAACATGCTTTCTTGCTGGTCAAGAAACAGAGTCCCAAAGTAGCCTAC